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KMID : 0545119970070050360
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Journal of Microbiology and Biotechnology
1997 Volume.7 No. 5 p.360 ~ p.362
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Isolation and Characterization of β-Hydroxybutyrate Dehydrogenase-deficient Mutant of Rhodobacter sphaeroides 2.4.1
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Kho Dohng-Hyo
Lee Jeong-Kug
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Abstract
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A transposon Tn5 mutant of Rhodobacter sphaeroides 2.4.1 was isolated for its impaired ability of growth on minimal medium containing β-hydroxybutyric acid as a sole carbon source. The mutant, R. sphaeroides S7 showed approximately 6-fold decrease in β-hydroxybutyrate dehydrogenase activity compared with that of wild type. In R. sphaeroides S7 the Tn5 was located in DNA region corresponding to a 4.2-kb EcoRI DNA fragment of R. sphaeroides 2.4.1 chromosome.
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